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KMID : 0545120110210090968
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 9 p.968 ~ p.971
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Mannitol Production by Leuconostoc citreum KACC 91348P Isolated from Kimchi
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Gan-Erdene Otgonbayar
Eom Hyun-Ju
Kim Beom-Soo
Ko Jae-Hyung
Han Nam-Soo
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Abstract
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Leuconostoc genus, which comprise heterofermentative lactic acid bacteria, reduces fructose to mannitol by recycling intracellular NADH. To evaluate the mannitol productivities of different Leuconostoc species, 5 stock cultures and 4 newly isolated strains were cultivated in MRS and simplified media containing glucose and fructose (1:2 ratio). Among them, L. citreum KACC 91348P, which was isolated from kimchi, showed superior result in cell growth rate, mannitol production rate, and yield in both media. The optimal condition for mannitol production of this strain was pH 6.5 and 30oC. When L. citreum KACC was cultured in simplified medium in a 2 l batch fermenter under optimal conditions, the maximum volumetric productivity was 14.83 g·l-1h-1 and overall yield was 86.6%. This strain is a novel and efficient mannitol producer originated from foods to be used for fermentation of fructose-containing foods.
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KEYWORD
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D-mannitol, Leuconostoc species, volumetric mannitol productivity, yield mol%
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